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Patogeneza i diagnostyka botulizmu

Elżbieta Kukier, *Janusz Dębski, Tomasz Grenda, Magdalena Goldsztejn, Krzysztof Kwiatek

 Zakład Higieny Pasz, Państwowy Instytut Weterynaryjny – Państwowy Instytut Badawczy,

Al. Partyzantów 57, 24-100 Puławy,

*Środowiskowe Laboratorium Spektrometrii Mas, Instytut Biochemii i Biofizyki, PAN,

ul. Pawińskiego 5A, 02-106 Warszawa

Streszczenie. Celem artykułu był opis struktury toksyn botulinowych, ich aktywności w organizmie oraz charakterystyka objawów botulizmu. Przedstawiono także laboratoryjne metody diagnostyczne botulizmu, uwzględniając ich czułość i przydatność w rutynowej diagnostyce choroby. Jedną z opisywanych metod diagnostycznych jest metoda wykrywania toksyn botulinowych oparta o technikę LC-MS/MS, stosowana do badań naukowych w Zakładzie Higieny Pasz PIWet-PIB Puławy. Uzyskane dotychczas wyniki badań w tym obszarze pokazują, że metoda ta ma ogromną wartość aplikacyjną oraz istotnie poprawi laboratoryjną diagnostykę botulizmu w przyszłości.

Słowa kluczowe: botulizm, toksyny botulinowe, wykrywanie, BoNT. 

 

Pathogenesis  and diagnostics of botulism

Elżbieta Kukier, *Janusz Dębski, Tomasz Grenda, Magdalena Goldsztejn, Krzysztof Kwiatek

Zakład Higieny Pasz, Państwowy Instytut Weterynaryjny – Państwowy Instytut Badawczy, Al. Partyzantów 57, 24-100 Puławy,

*Środowiskowe Laboratorium Spektrometrii Mas, Instytut Biochemii i Biofizyki, PAN, ul. Pawińskiego 5A, 02-106 Warszawa

Summary. The aim of study was characterization of botulinum toxins and their action on protein complex (SNARE) in presynaptic membrane as well as symptoms of botulism. In this article, the characterization of methods for laboratory diagnostics of botulism, taking into account their application value, was also demonstrated. Moreover, the latest achievements of the Department of Hygiene of Animal Feedingstuffs (NVRI, Pulawy) in development and implementation of LC-MS/MS method into laboratory practice, have been presented. According to the authors of the study, the usage of this method has a great application significance, and will improve the laboratory diagnostics of botulism in the future.

Keywords: botulinum toxins, botulism, detection, BoNT. 

 

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